GeneProof Hepatitis C Virus (HCV) PCR Kit
Kat.: HCV/PLUS/100
A termék CE/IVD minősítéssel rendelkezik és az OGYÉI által nyilvántartásba vett.
A HCV 1–7 genotípus detektálására alkalmas, ‘Ready-To-Use’ master mix. Az 5′ UTR konzervatív régió szekvenciát célozza. (100 reakció)
Leírás
PRODUCT ADVANTAGES
DETERMINES A BROAD SPECTRUM OF HCV GENOTYPES
- The kit determines HCV genotypes 1 – 7
SINGLE TUBE READY-TO-USE MASTERMIX
- Contains all components for PCR amplification
- No additional PCR reagents pipetting necessary
- Reduces the need for qualified laboratory staff
- Ensures reproducibility of the results
INTERNAL RNA CONTROL
- Controls the whole process
- RNA extraction, reverse transcription and PCR amplification
- IC is included in the PCR Kit
COMPATIBLE WITH A WIDE RANGE OF REAL-TIME PCR DEVICES
Technical specification
Intended User | For professional use in laboratories with trained staff |
Technology | real-time PCR |
Target Analysis | qualitative and quantitative |
Target Sequence | conservative region of 5′ UTR sequence |
Analytical Specificity | HCV genotype 1–8, 100 % |
Analytical Sensitivity (LoD) | Reaches up to 7.87 IU/ml with the probability of 95% (on AcroMetrix HCV High Control using automatic extraction MagCore NA Extractor) Reaches up to 208.54 IU/ml with the probability of 95% (on AcroMetrix HCV High Control using automatic extraction croBEE NA16 Nucleic Acid Extraction System) Reaches up to 213.965 IU/ml with the probability of 95% (on HCV NIBSC 14/150 using manual extraction GeneProof PathogenFree RNA Isolation Kit) |
Linear Range | 1010 – 25 IU/ml with precision of ± 0.5 log |
Dynamic Range | 1010 – 7.87 IU/ml |
Reporting Units | IU/μl (1 IU = 5.62 cp) |
Validated Specimen | Plasma, serum |
Storage | -20 ± 5 °C |
Validated Extraction Methods | croBEE NA16 Nucleic Acid Extraction System GeneProof PathogenFree RNA Isolation Kit |
Instruments | croBEE Real-Time PCR System* Applied Biosystems 7300 / 7500 Real-Time PCR System AriaMx Real-Time PCR System CFX Connect™ / CFX96™/ Dx Real-Time PCR Detection System DT lite Real-Time PCR System LightCycler® 2.0 / 480 LineGene 9600 / 9600 Plus Rotor-Gene 3000 / 6000 / Q SLAN® Real-Time PCR System |
Required Detection Channels | FAM, HEX |