GeneProof VRE PCR Kit
Kat.: VRE/ISEX/025
A termék CE/IVD minősítéssel rendelkezik és az OGYÉI által nyilvántartásba vett.
A vanA és vanB detektálására és elkülönítésére alkalmas, ‘Ready-To-Use’ master mix. Nincs szükség előzetes tenyésztésre. (25 reakció)
Leírás
PRODUCT ADVANTAGES
DIRECT DETECTION FROM CLINICAL MATERIAL
- No need of previous cultivation
- Quick result from primary clinical material
UNIVERSAL PCR PROFILE
- Easily combinable with e.g. GeneProof MRSA PCR Kit
IDENTIFICATION OF vanA AND vanB
- Detects and distinguishes vanA and vanB genes
- Enables choosing appropriate therapy
CONTAMINATION ELIMINATION
- Ready-to-Use Master Mix contains Uracil-DNA glycosylase (UNG) and dUTPs eliminating contamination with amplification products
COMPATIBLE WITH A WIDE RANGE OF REAL-TIME PCR DEVICES
CE IVD CERTIFIED DIAGNOSTIC TEST
Technical specification
| Indication | in vitro diagnostic medical device |
| Intended User | For professional use in laboratories with trained staff |
| Technology | real-time PCR |
| Type of Analysis | qualitative |
| Target Sequence | specific conservative DNA sequence in the area of the vanA and vanB genes |
| Specificity | vanA, vanB genes (e. g. Enterococcus faecalis, Enterococcus faecium, Enterococcus gallinarium, etc.), 100 % |
| Analytical Sensitivity (LoD) | for vanA reaches 1.398 cp/μl with the probability of 95 % for vanB reaches 1.026 cp/μl with the probability of 95 % |
| Extraction/Inhibition Control | PCR inhibition and DNA extraction efficiency control |
| Validated Specimen | whole blood, sputum, stool, urine |
| Storage | -20 ± 5 °C |
| Validated Extraction Methods | croBEE NA16 Nucleic Acid Extraction System GeneProof PathogenFree DNA Isolation Kit |
| Validated Instruments | croBEE Real-Time PCR System* Applied Biosystems 7500 Real-Time PCR System AriaMx Real-Time PCR System CFX96™ / Dx Real-Time PCR Detection System LightCycler® 480 LineGene 9600 / 9600 Plus Mic qPCR Cycler QuantStudio™ 5 Real-Time PCR System Rotor-Gene 3000 / Q SLAN® Real-Time PCR System |
| Required Detection Channels | FAM, HEX, Cy5 |
| External Quality Assessment | regularly tested by QCMD and Instand e.V. External Quality Assessment panels – results at www.geneproof.com |
| Certification | CE IVD |
*Validated instruments
